The Attitude Control System Concept for the Joint Australian Engineering Micro-Satellite (JAESat)

JAESat is a joint micro-satellite project between Queensland University of Technology (QUT), Australian Space Research Institute (ASRI) and other national and international partners including the Australian Cooperative Research Centre for Satellite Systems (CRCSS), Kayser-Threde GmbH, Aerospace Concepts and Auspace who will contribute to this project. The JAESat micro-satellite project is an educational and GNSS technology demonstration mission. The main objectives of the JAESat mission are the design and development of a micro-satellite in order to educate and train students and also to generate a platform in space for technology demonstration and conduction of research on a low-cost basis. The main payload on-board JAESat will be a GPS receiver called SPARx (SPace Applications Receiver), developed by the Queensland University of Technology for attitude and orbit determination. In addition to the GPS based attitude sensor, a star sensor will be on-board JAESat for attitude determination. JAESat will be three-axis stabilized based on a zero-momentum approach using magnetic coil actuators. This paper will outline the Attitude Control System (ACS) concept for JAESat including: subsystem configuration and components, performance requirements, control mode definition, attitude dynamic modeling, control law development, and attitude determination concept. Performance of the JAESat ACS is predicted via simulations using a comprehensive ACS model developed in Matlab Simulink

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. For example, a key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process versus those that measure fl ux through the autophagy pathway (i.e., the complete process including the amount and rate of cargo sequestered and degraded). In particular, a block in macroautophagy that results in autophagosome accumulation must be differentiated from stimuli that increase autophagic activity, defi ned as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (inmost higher eukaryotes and some protists such as Dictyostelium ) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the fi eld understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. It is worth emphasizing here that lysosomal digestion is a stage of autophagy and evaluating its competence is a crucial part of the evaluation of autophagic flux, or complete autophagy. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. Along these lines, because of the potential for pleiotropic effects due to blocking autophagy through genetic manipulation it is imperative to delete or knock down more than one autophagy-related gene. In addition, some individual Atg proteins, or groups of proteins, are involved in other cellular pathways so not all Atg proteins can be used as a specific marker for an autophagic process. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field

Robust adaptive control of rigid spacecraft attitude maneuvers

In this thesis novel feedback attitude control algorithms and attitude estimation algorithms are developed for a three-axis stabilised spacecraft attitude control system. The spacecraft models considered include a rigid-body spacecraft equipped with (i) external control torque devices, and (ii) a redundant reaction wheel configuration. The attitude sensor suite comprises a three-axis magnetometer and three-axis rate gyroscope assembly. The quaternion parameters (also called Euler symmetric parameters), which globally avoid singularities but are subject to a unity-norm constraint, are selected as the primary attitude coordinates. There are four novel contributions presented in this thesis. The first novel contribution is the development of a robust control strategy for spacecraft attitude tracking maneuvers, in the presence of dynamic model uncertainty in the spacecraft inertia matrix, actuator magnitude constraints, bounded persistent external disturbances, and state estimation error. The novel component of this algorithm is the incorporation of state estimation error into the stability analysis. The proposed control law contains a parameter which is dynamically adjusted to ensure global asymptotic stability of the overall closedloop system, in the presence of these specific system non-idealities. A stability proof is presented which is based on Lyapunov's direct method, in conjunction with Barbalat's lemma. The control design approach also ensures minimum angular path maneuvers, since the attitude quaternion parameters are not unique. The second novel contribution is the development of a robust direct adaptive control strategy for spacecraft attitude tracking maneuvers, in the presence of dynamic model uncertainty in the spacecraft inertia matrix. The novel aspect of this algorithm is the incorporation of a composite parameter update strategy, which ensures global exponential convergence of the closed-loop system. A stability proof is presented which is based on Lyapunov's direct method, in conjunction with Barbalat's lemma. The exponential convergence results provided by this control strategy require persistently exciting reference trajectory commands. The control design approach also ensures minimum angular path maneuvers. The third novel contribution is the development of an optimal control strategy for spacecraft attitude maneuvers, based on a rigid body spacecraft model including a redundant reaction wheel assembly. The novel component of this strategy is the proposal of a performance index which represents the total electrical energy consumed by the reaction wheel over the maneuver interval. Pontraygin's minimum principle is applied to formulate the necessary conditions for optimality, in which the control torques are subject to timevarying magnitude constraints. The presence of singular sub-arcs in the statespace and their associated singular controls are investigated using Kelley's necessary condition. The two-point boundary-value problem (TPBVP) is formulated using Pontrayagin's minimum principle. The fourth novel contribution is an attitude estimation algorithm which estimates the spacecraft attitude parameters and sensor bias parameters from three-axis magnetometer and three-axis rate gyroscope measurement data. The novel aspect of this algorithm is the assumption that the state filtering probability density function (PDF) is Gaussian distributed. This Gaussian PDF assumption is also applied to the magnetometer measurement model. Propagation of the filtering PDF between sensor measurements is performed using the Fokker-Planck equation, and Bayes theorem incorporates measurement update information. The use of direction cosine matrix elements as the attitude coordinates avoids any singularity issues associated with the measurement update and estimation error covariance representation

Spacecraft attitude maneuvers using composite adaptive control with invariant sliding manifold

An adaptive control strategy is developed for spacecraft attitude tracking maneuvers, in the presence of structured uncertainty in the spacecraft inertia parameters. The proposed strategy incorporates a composite parameter update law, which guarantees global asymptotic stability of the overall closed-loop system. A stability analysis is performed using Lyapunov's direct method, in conjunction with Barbalat's lemma. The asymptotic convergence results provided by this control strategy require persistently exciting reference trajectory commands. Simulation results are provided which demonstrate the performance of the proposed adaptive control design for a rigid spacecraft tracking a time-varying reference trajectory

Safety and immunogenicity of the ChAdOx1 nCoV-19 vaccine against SARS-CoV-2: a preliminary report of a phase 1/2, single-blind, randomised controlled trial.

BACKGROUND: The pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) might be curtailed by vaccination. We assessed the safety, reactogenicity, and immunogenicity of a viral vectored coronavirus vaccine that expresses the spike protein of SARS-CoV-2. METHODS: We did a phase 1/2, single-blind, randomised controlled trial in five trial sites in the UK of a chimpanzee adenovirus-vectored vaccine (ChAdOx1 nCoV-19) expressing the SARS-CoV-2 spike protein compared with a meningococcal conjugate vaccine (MenACWY) as control. Healthy adults aged 18-55 years with no history of laboratory confirmed SARS-CoV-2 infection or of COVID-19-like symptoms were randomly assigned (1:1) to receive ChAdOx1 nCoV-19 at a dose of 5 × 1010 viral particles or MenACWY as a single intramuscular injection. A protocol amendment in two of the five sites allowed prophylactic paracetamol to be administered before vaccination. Ten participants assigned to a non-randomised, unblinded ChAdOx1 nCoV-19 prime-boost group received a two-dose schedule, with the booster vaccine administered 28 days after the first dose. Humoral responses at baseline and following vaccination were assessed using a standardised total IgG ELISA against trimeric SARS-CoV-2 spike protein, a muliplexed immunoassay, three live SARS-CoV-2 neutralisation assays (a 50% plaque reduction neutralisation assay [PRNT50]; a microneutralisation assay [MNA50, MNA80, and MNA90]; and Marburg VN), and a pseudovirus neutralisation assay. Cellular responses were assessed using an ex-vivo interferon-γ enzyme-linked immunospot assay. The co-primary outcomes are to assess efficacy, as measured by cases of symptomatic virologically confirmed COVID-19, and safety, as measured by the occurrence of serious adverse events. Analyses were done by group allocation in participants who received the vaccine. Safety was assessed over 28 days after vaccination. Here, we report the preliminary findings on safety, reactogenicity, and cellular and humoral immune responses. The study is ongoing, and was registered at ISRCTN, 15281137, and ClinicalTrials.gov, NCT04324606. FINDINGS: Between April 23 and May 21, 2020, 1077 participants were enrolled and assigned to receive either ChAdOx1 nCoV-19 (n=543) or MenACWY (n=534), ten of whom were enrolled in the non-randomised ChAdOx1 nCoV-19 prime-boost group. Local and systemic reactions were more common in the ChAdOx1 nCoV-19 group and many were reduced by use of prophylactic paracetamol, including pain, feeling feverish, chills, muscle ache, headache, and malaise (all p<0·05). There were no serious adverse events related to ChAdOx1 nCoV-19. In the ChAdOx1 nCoV-19 group, spike-specific T-cell responses peaked on day 14 (median 856 spot-forming cells per million peripheral blood mononuclear cells, IQR 493-1802; n=43). Anti-spike IgG responses rose by day 28 (median 157 ELISA units [EU], 96-317; n=127), and were boosted following a second dose (639 EU, 360-792; n=10). Neutralising antibody responses against SARS-CoV-2 were detected in 32 (91%) of 35 participants after a single dose when measured in MNA80 and in 35 (100%) participants when measured in PRNT50. After a booster dose, all participants had neutralising activity (nine of nine in MNA80 at day 42 and ten of ten in Marburg VN on day 56). Neutralising antibody responses correlated strongly with antibody levels measured by ELISA (R2=0·67 by Marburg VN; p<0·001). INTERPRETATION: ChAdOx1 nCoV-19 showed an acceptable safety profile, and homologous boosting increased antibody responses. These results, together with the induction of both humoral and cellular immune responses, support large-scale evaluation of this candidate vaccine in an ongoing phase 3 programme. FUNDING: UK Research and Innovation, Coalition for Epidemic Preparedness Innovations, National Institute for Health Research (NIHR), NIHR Oxford Biomedical Research Centre, Thames Valley and South Midland's NIHR Clinical Research Network, and the German Center for Infection Research (DZIF), Partner site Gießen-Marburg-Langen

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field